HEPES, Free Acid
HEPES is a zwitterionic buffer used to maintain pH of media used in cell cultures. It is one of Good’s twelve buffers that has a pKa value similar to its pH value making it an ideal buffer for pH maintenance. It has only one restriction, it interferes with the Folin protein assay. This buffer can form radicals so it is not suitable for redox studies. HEPES is used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water. These are general specifications. BioBuffer Solutions will customize our products to meet your quality based requirements.
Product Specifications
| ANALYSIS | SPECIFICATIONS |
|---|---|
|
250 nm Absorbance (0.1M) 260 nm 280 nm |
0.060 a.u. max. 0.060 a.u. max. 0.080 a.u. max. |
| Appearance and Color | White / Crystals |
| Assay | 99.0% min. |
| Identification (IR) | Passes Test |
| pH (5% Soln.) | 5.0 – 6.5 |
| Residue on Ignition | 0.2% max. |
| Solubility (5%) | Passes Test |
Printable Version
General Product Description:
- HEPES is a White Crystalline product.
- Molecular Formula: C8H18N2O4S
- Molecular Weight: 238.30 g/mol.
- CAS Number: 7365-45-9.
- Synonyms: N-(2-Hydroxyethyl)piperazine-N’-2-ethanesulfonic acid, 2-[4-(2 hydroxyethyl)piperazin-1-yl]ethanesulfonic acid.
Intended Use Statement:
Material represented by this grade is suitable to be used only as the following: Quality System Manufactured Process Chemical for use in further manufacturing or as a reagent for laboratory and research. The material represented by this grade is not suitable to be used as an Excipient, Active Pharmaceutical Ingredient, Drug, Drug Product or household item.
Expiration/Retest Date:
BioBuffer Solutions does not assign expiration or retest dates for Bio Ultra Grade materials.
Storage and Shipping Conditions:
Ship and Store in ambient temperature.
Package Sizes:
10kg, 25kg and 50kg pails.
Bulk GMP Fine Chemicals exclusively manufactured in North America
American Manufactured - FDA Registered GMP Facilities: Parenteral Excipients, GMP Small Molecule Intermediates, GMP Biological Buffers, Parenteral Carbohydrates, GMP Chlorinated Amino Acids, GMP Solutions in Sterile Single Use Packaging and other Reagent & GMP Fine Chemicals.