HEPES, Reagent

BioBuffer Solutions Brand Product

Reagent

HEPES

Endotoxin Tested

Product Code: HEPE-5251

*Bioburden and Endotoxin Tested

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Product Code: HEPE-5251

Suitable for use in biological and biotech chemical process applications from R&D through scale production.

HEPES is a zwitterionic buffer used to maintain pH of media used in cell cultures. It is one of Good’s buffers that has a pKa value similar to its pH value, making it an ideal buffer for pH maintenance. A known limitation is its interference with the Folin protein assay. This buffer can form radicals, so it is not suitable for redox studies. HEPES is a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.

CAS #: 7365-45-9
Molecular Formula: C₈H₁₈N₂O₄S
F.W.: 238.31 g/mol

Product Specifications

Analysis	Specifications
Assay (by Titration, Dried Basis)	> = 99.0%
pH (5% at 25^◦C)	5.0 - 6.5
Loss on Drying (105^◦C, 3 hours)	< = 1.0%
UV Absorbance (260nm, 1mol/L, 1cm)	< = 0.1
UV Absorbance (280nm, 1mol/L, 1cm)	< = 0.1
Iron	< = 0.0005%
Sulfate	< = 0.05%
Heavy Metals (as Pb)	< = 0.0005%
Endotoxin, 0.2% Solution	< = 0.10 EU/mL
Sulfated Ash (600^◦C)	< = 0.2%
pKa at 20^◦C	7.35 - 7.69
pKa at 25^◦C	7.2 - 7.8
Solubility (1mol/L, Soln. in H₂O)	Clear, Complete and Colorless
Identification	IR Matches Reference
Appearance	White Crystalline Powder

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Key Product Features:

Appears as a white crystalline powder
Contains no known major food allergens (as defined by FDA and WHO)
The final product and its raw materials are not derived from nor come into contact with animal parts, animal products, and/or animal byproducts or derivatives.
Is not subject to genetic modification
Synonyms: N-(2-Hydroxyethyl) Piperazine-N'-2-Ethanesulfonic Acid; 4-(2-Hydroxyethyl) Piperazine-1-Ethanesulfonic Acid

Shelf-Life Policy:

Typical shelf-life for this material is a two-year retest date with a three-year expiry from the date of manufacturing or repackaging. Please inquire for further information.

Storage & Shipping:

Refer to SDS.

Package Sizes:

1kg, 5kg, 10kg, 25kg, and 50kg

Bulk GMP Fine Chemicals exclusively manufactured in North America

American Manufactured - FDA Registered GMP Facilities: Parenteral Excipients, GMP Small Molecule Intermediates, GMP Biological Buffers, Parenteral Carbohydrates, GMP Chlorinated Amino Acids, GMP Solutions in Sterile Single Use Packaging and other Reagent & GMP Fine Chemicals.